Transcript levels and metabolic fluxes in S. cerevisiae Role of Transcriptional Regulation in Controlling Fluxes in Central Carbon Metabolism of Saccharomyces cerevisiae, a Chemostat Culture Study
نویسندگان
چکیده
Kluyver Laboratory of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands Beijerinck Laboratory, DSM-GIST Delft, PO Box 1, 2600 MA Delft, The Netherlands Corresponding author: P. Daran-Lapujade, Kluyver Laboratory of Biotechnology, Delft University of Technology, Julianalaan 67, NL-2628 BC Delft, The Netherlands. Telephone +31 15 278 2410; Fax +31 15 278 2355; E-mail [email protected]
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Role of transcriptional regulation in controlling fluxes in central carbon metabolism of Saccharomyces cerevisiae. A chemostat culture study.
In contrast to batch cultivation, chemostat cultivation allows the identification of carbon source responses without interference by carbon-catabolite repression, accumulation of toxic products, and differences in specific growth rate. This study focuses on the yeast Saccharomyces cerevisiae, grown in aerobic, carbon-limited chemostat cultures. Genome-wide transcript levels and in vivo fluxes w...
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